Lab work Mondays

Check to see if BioRad bovine serum albumin (BSA) Protocol works with oyster tissue

link to BSA protocol

if it does, use the results from the assay run to calculate total protein concentration per sample (ug/mL)

I am going to want to use various dillutions of the samples so I can get the protein concentration in the linear range (<2000 ug/mL)

Next is to run the Citrate-Synthase assay with samples

Link to CS assay procedure

Pay special attention for bubbles present, use tabletop centrifuge, pipette more carefully

Run technical replicates on gill and mantle tissue

Written on October 8, 2021