Update for C.gigas Molecular Delivery Project w/ Mac Gavery on August 18th 2022
protocol here
Experimental Set-up (C. gigas embryos)
- Group A: Control, fertilized in 1.5mL tubes, densities of 5,000 cells/mL -> 20,000 cells/mL, no dextran (embryos)
- Group B: Dextran, same densityes as above, all componenets present (embryos)
- Group C: Mac’s Control, fertilized in hatchery style, oocytes with dextran, same densities as above (embryos)
1 hours post fertilization
| Group # |
Regular |
Fluorescence |
| Group A |
 |
 |
| Group B |
 |
 |
24 hours post fertilization (August 19th)
| Group # |
Regular |
Fluorescence |
| Group A |
 |
 |
| Group B |
 |
 |
48 hours post fertilization (August 20th)
- All densities have swimming larvae, no obvious deformities on average
- I would use a density of ~25,000 to allow for easy viewing under the scope, 5,000 was not dense enough for counts
| Group # |
Regular |
Fluorescence |
| Group A |
 |
 |
| Group B |
 |
 |
| Group C |
 |
 |